液相色谱中的化学发光检测：在临床、制药、环境和食品分析中的应用——综述

Chemiluminescence detection in liquid chromatography: applications to clinical, pharmaceutical, environmental and food analysis--a review.

作者信息

Gámiz-Gracia Laura, García-Campaña Ana M, Huertas-Pérez José F, Lara Francisco J

机构信息

Department of Analytical Chemistry, Faculty of Sciences, Campus Fuentenueva, University of Granada, E-18071 Granada, Spain.

出版信息

Anal Chim Acta. 2009 Apr 27;640(1-2):7-28. doi: 10.1016/j.aca.2009.03.017. Epub 2009 Mar 20.

DOI:10.1016/j.aca.2009.03.017

PMID:19362614

Abstract

Chemiluminescence (CL)-based detection has become in the last years quite a useful detecting tool in liquid chromatography (HPLC) due to its simplicity, low cost and high sensitivity and selectivity, and the development in instrumentation. Minimal instrumentation is required and no external light source is needed; thus, the optical system is quite simple. As a consequence, a wide variety of analytical methods have been developed in clinical, pharmaceutical, environmental and food analysis. In this review, applications of the HPLC-CL coupling in those different fields have been included and classified in relation to the different CL systems employed (namely peroxyoxalate reaction, tris(2,2'-bipyridine) ruthenium (II) reaction, luminol system and direct oxidations) and also sub-classified according to the group of analyte. The review covers the literature from 2000 until the end of 2008.

摘要

近年来，基于化学发光（CL）的检测方法因其操作简单、成本低、灵敏度和选择性高以及仪器设备的发展，已成为液相色谱（HPLC）中一种非常有用的检测工具。所需仪器最少，无需外部光源；因此，光学系统非常简单。结果，在临床、制药、环境和食品分析领域开发了各种各样的分析方法。在本综述中，已纳入了HPLC-CL联用在这些不同领域的应用，并根据所采用的不同CL系统（即过氧草酸酯反应、三（2,2'-联吡啶）钌（II）反应、鲁米诺体系和直接氧化）进行分类，还根据分析物类别进行了细分。该综述涵盖了2000年至2008年底的文献。