整合子的首个基因盒作为在宏基因组中寻找适应性基因的靶点。
First gene cassettes of integrons as targets in finding adaptive genes in metagenomes.
作者信息
Huang Lionel, Cagnon Christine, Caumette Pierre, Duran Robert
机构信息
Université de Pau et des Pays de l'Adour, IPREM UMR CNRS, IBEAS, BP, Pau, France.
出版信息
Appl Environ Microbiol. 2009 Jun;75(11):3823-5. doi: 10.1128/AEM.02394-08. Epub 2009 Apr 10.
Abstract
The first gene cassettes of integrons are involved in the last adaptation response to changing conditions and are also the most expressed. We propose a rapid method for the selection of clones carrying an integron first gene cassette that is useful for finding adaptive genes in environmental metagenomic libraries.
摘要
整合子的首个基因盒参与了对不断变化条件的最终适应性反应，且也是表达量最高的。我们提出了一种快速方法，用于筛选携带整合子首个基因盒的克隆，该方法有助于在环境宏基因组文库中寻找适应性基因。
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