Dnistrian A M, Skipski V P, Barclay M, Stock C C
Cancer Res. 1977 Jul;37(7 Pt 1):2182-7.
Neutral glycosphingolipids and gangliosides were quantified in lipid extracts from normal rat liver and Morris hepatoma 5123TC and their isolated plasma membranes to determine differences in these components in the cell surface membranes of malignant cells. Glycosphingolipids present in rat liver and hepatoma were concentrated in plasma membranes, and glycosphingolipid patterns in plasma membranes reflected those of their respective whole cells. Neutral glycospingolipids of plasma membranes from normal liver and from hepatoma consisted of ceramide mono- and dihexosides, together accounting for 83 to 86% of the total neutral glycosphingolipids and some ceramide tri- and tetrahexosides. In plasma membranes from hepatoma, the concentration of neutral glycosphingolipids was generally greater than in plasma membranes from normal liver. Gangliosides of plasma membranes from hepatoma were altered more drastically, since trisialogangliosides, present in plasma membranes from normal liver, were absent, while hematosides, monosialogangliosides, and disialogangliosides were increased an average eight-fold. These data are compatible with a concept of incomplete synthesis of trisialogangliosides in hepatoma and an accumulation precursor gangliosides.
对正常大鼠肝脏和莫里斯肝癌5123TC及其分离的质膜的脂质提取物中的中性糖鞘脂和神经节苷脂进行了定量分析，以确定恶性细胞质膜中这些成分的差异。大鼠肝脏和肝癌中存在的糖鞘脂集中在质膜中，质膜中的糖鞘脂模式反映了其各自全细胞的模式。正常肝脏和肝癌质膜中的中性糖鞘脂由神经酰胺单己糖苷和二己糖苷组成，两者合计占总中性糖鞘脂的83%至86%，还有一些神经酰胺三己糖苷和四己糖苷。在肝癌质膜中，中性糖鞘脂的浓度通常高于正常肝脏质膜。肝癌质膜中的神经节苷脂变化更为显著，因为正常肝脏质膜中存在的三唾液酸神经节苷脂不存在，而血型糖苷、单唾液酸神经节苷脂和双唾液酸神经节苷脂平均增加了八倍。这些数据与肝癌中三唾液酸神经节苷脂合成不完全以及前体神经节苷脂积累的概念相符。
