Wood R, Upreti G C, deAntueno R J
Lipids. 1986 Apr;21(4):292-300. doi: 10.1007/BF02536416.
Subcellular fractions of nuclei, mitochondria, endoplasmic reticulum, plasma membrane and cytosol were prepared from liver and hepatoma 7288CTC. Marker enzyme activities, biochemical compositions and electron microscopy were used to establish purity. Hepatoma NADH: cytochrome C reductase and 5'-nucleotidase exhibited abnormal subcellular distributions. The lipids from the subcellular fractions were examined in detail. Mitochondria and plasma membranes were characterized by elevated percentages of diphosphatidylglycerol and sphingomyelin, respectively, in both tissues. All hepatoma subcellular fractions contained dramatically elevated levels of sphingomyelin and cholesterol, two components that form preferential strong complexes in vitro. The fatty acid composition of hepatoma sphingomyelin differed markedly from liver and, unlike liver, did not exhibit organelle specific compositions. Some hepatoma lipid classes contained reduced percentages of palmitate while others contained higher levels. Hepatoma phosphatidylcholine and phosphatidylethanolamine from organelles contained lower percentages of long chain polyunsaturated fatty acids than liver. Generally, unique fatty acid profiles exhibited by individual phospholipid classes of liver subcellular fractions were absent or much reduced in the hepatoma. The ratios of oleate to vaccenate were near one for most of the phospholipid classes of most liver fractions, but all hepatoma classes, with few exceptions, contained a much higher percentage of oleate in all subcellular fractions. The hypothesis is proposed that the origin of some acyl moieties for the biosynthesis of various hepatoma lipid classes differs from liver sources. The possible changes in acyl pools, sources and compartments for complex lipid biosynthesis could result in change in the quantities of molecular species that could contribute to the abnormal properties of the hepatoma membranes.
从肝脏和肝癌7288CTC中制备细胞核、线粒体、内质网、质膜和胞质溶胶的亚细胞组分。通过标记酶活性、生化组成和电子显微镜来确定纯度。肝癌中的NADH：细胞色素C还原酶和5'-核苷酸酶表现出异常的亚细胞分布。对亚细胞组分中的脂质进行了详细检查。在这两种组织中，线粒体和质膜的特征分别是二磷脂酰甘油和鞘磷脂的百分比升高。所有肝癌亚细胞组分中鞘磷脂和胆固醇的水平都显著升高，这两种成分在体外形成优先强复合物。肝癌鞘磷脂的脂肪酸组成与肝脏明显不同，并且与肝脏不同，它没有表现出细胞器特异性组成。一些肝癌脂质类别中棕榈酸的百分比降低，而另一些则含有更高水平。来自细胞器的肝癌磷脂酰胆碱和磷脂酰乙醇胺中长链多不饱和脂肪酸的百分比低于肝脏。一般来说，肝癌中缺乏或大大减少了肝脏亚细胞组分中个别磷脂类所表现出的独特脂肪酸谱。大多数肝脏组分中大多数磷脂类的油酸与反式油酸的比例接近1，但除少数例外，所有肝癌类别在所有亚细胞组分中油酸的百分比都高得多。提出的假设是，各种肝癌脂质类生物合成的一些酰基部分的来源与肝脏来源不同。复合脂质生物合成中酰基库、来源和区室的可能变化可能导致分子种类数量的变化，这可能导致肝癌细胞膜的异常特性。
