Zhang Yongfei, Jung Chun Suk, De Jong Walter S
Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853-1901, USA.
Theor Appl Genet. 2009 Jun;119(1):143-50. doi: 10.1007/s00122-009-1024-3. Epub 2009 Apr 11.
Interest in anthocyanin-pigmented potato tuber flesh is increasing. To genetically map and characterize loci that influence this trait, diploid potato clone 10618-01, which has partially pigmented flesh, was crossed with diploid 320-02, which has white flesh. Almost all progeny exhibited purple coloration in the flesh, with some clones having only a small percentage of tissue pigmented, other clones having most tissue pigmented, and the majority of clones showing intermediate color phenotypes. The two parents and 228 progeny were genotyped with 493 AFLP, 8 CAPS, and 13 SSR markers. QTLs influencing extent of flesh pigmentation were detected on chromosomes 5, 8, and 9. The potato homolog of Petunia an1, a basic helix-loop-helix (bHLH) transcriptional regulator of anthocyanin biosynthesis, was found to co-localize with the QTL on chromosome 9. A CAPS marker based on this gene was used to evaluate a collection of 21 tetraploid potato clones with highly or fully pigmented red or purple flesh, as well as 53 cultivars with white or yellow flesh. All 21 pigmented-flesh clones shared a marker allele that was present in only 21 of the 53 white and yellow clones, suggesting that a common bHLH allele contributes toward, although it is clearly not sufficient for, highly or fully pigmented tuber flesh in cultivated potato.
对花青素色素沉着的马铃薯块茎果肉的研究兴趣日益增加。为了对影响该性状的基因座进行遗传定位和表征，将具有部分色素沉着果肉的二倍体马铃薯克隆10618 - 01与具有白色果肉的二倍体320 - 02进行杂交。几乎所有后代的果肉都呈现紫色，一些克隆只有一小部分组织有色素沉着，其他克隆大部分组织有色素沉着，大多数克隆表现出中间颜色表型。使用493个AFLP、8个CAPS和13个SSR标记对两个亲本和228个后代进行基因分型。在5号、8号和9号染色体上检测到影响果肉色素沉着程度的QTL。矮牵牛an1的马铃薯同源物，一种花青素生物合成的基本螺旋-环-螺旋（bHLH）转录调节因子，被发现与9号染色体上的QTL共定位。基于该基因的CAPS标记用于评估21个具有高度或完全色素沉着的红色或紫色果肉的四倍体马铃薯克隆以及53个具有白色或黄色果肉的品种。所有21个有色素沉着果肉的克隆都共享一个标记等位基因，该等位基因仅在53个白色和黄色克隆中的21个中出现，这表明一个共同的bHLH等位基因对栽培马铃薯中高度或完全色素沉着的块茎果肉有贡献，尽管显然并不充分。
