Liu Yuhui, Li Yuanming, Liu Zhen, Wang Lei, Lin-Wang Kui, Zhu Jinyong, Bi Zhenzhen, Sun Chao, Zhang Junlian, Bai Jiangping
State Key Laboratory of Aridland Crop Science/Agronomy College, Gansu Agricultural University, Lanzhou 730070, China.
College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
iScience. 2022 Dec 29;26(2):105903. doi: 10.1016/j.isci.2022.105903. eCollection 2023 Feb 17.
Potatoes consist of flavonoids that provide health benefits for human consumers. To learn more about how potato tuber flavonoid accumulation and flesh pigmentation are controlled, we analyzed the transcriptomic and metabolomic profile of potato tubers from three colored potato clones at three developmental phases using an integrated approach. From the 72 flavonoids identified in pigmented flesh, differential abundance was noted for anthocyanins, flavonols, and flavones. Weighted gene co-expression network analysis further allowed modules and candidate genes that positively or negatively regulate flavonoid biosynthesis to be identified. Furthermore, an R2R3-MYB repressor StMYB3 and an R3-MYB repressor StMYBATV involved in the modulation of anthocyanin biosynthesis during tuber development were identified. Both StMYB3 and StMYBATV could interact with the cofactor StbHLH1 and repress anthocyanin biosynthesis. Our results indicate a feedback regulatory mechanism of a coordinated MYB activator-repressor network on fine-tuning of potato tuber pigmentation during tuber development.
土豆含有类黄酮，对人类消费者有益健康。为了更深入了解马铃薯块茎类黄酮积累和果肉色素沉着是如何被调控的，我们采用综合方法分析了三个彩色马铃薯克隆在三个发育阶段的块茎转录组和代谢组图谱。在有色果肉中鉴定出的72种类黄酮中，花青素、黄酮醇和黄酮的丰度存在差异。加权基因共表达网络分析进一步确定了正向或负向调节类黄酮生物合成的模块和候选基因。此外，还鉴定出了在块茎发育过程中参与花青素生物合成调控的R2R3-MYB抑制因子StMYB3和R3-MYB抑制因子StMYBATV。StMYB3和StMYBATV都能与辅因子StbHLH1相互作用并抑制花青素生物合成。我们的结果表明，在块茎发育过程中，MYB激活因子-抑制因子网络协同对马铃薯块茎色素沉着进行微调存在一种反馈调节机制。
