Naito Yuji, Yoshikawa Toshikazu
Forum Nutr. 2009;61:39-54. doi: 10.1159/000212737. Epub 2009 Apr 7.
In lifestyle-related diseases including metabolic syndrome, atherosclerosis, and cancer, oxidative stress is indicated by several markers, among which are lipid peroxides, aldehydes, and nitrotyrosine. We hypothesized that identification of proteins that are posttranslationally modified due to oxidative stress would lead to a greater understanding of some of the potential molecular mechanisms involved in degeneration and inflammation in these disorders. Proteomics is an emerging method for identification of proteins and their modification residues, and its application to food factor science is just beginning. Especially, we can obtain several monoclonal antibodies to detect specifically oxidized proteins, which can be applied to analysis by immunostaining or immunoblot. In this review, we present the use of these monoclonal antibodies in several diseases, from which new insights have emerged into mechanisms of metabolism and inflammation in these disorders that are associated with oxidative stress.
在包括代谢综合征、动脉粥样硬化和癌症在内的与生活方式相关的疾病中，氧化应激由多种标志物指示，其中包括脂质过氧化物、醛类和硝基酪氨酸。我们推测，鉴定因氧化应激而发生翻译后修饰的蛋白质，将有助于更深入地了解这些疾病中涉及退化和炎症的一些潜在分子机制。蛋白质组学是一种用于鉴定蛋白质及其修饰残基的新兴方法，其在食品因子科学中的应用才刚刚开始。特别是，我们可以获得几种单克隆抗体来特异性检测氧化蛋白质，这些抗体可用于免疫染色或免疫印迹分析。在这篇综述中，我们介绍了这些单克隆抗体在几种疾病中的应用，从中对与氧化应激相关的这些疾病的代谢和炎症机制有了新的认识。
