Piao Xiang Lan, Cho Eun Jung, Jang Moon Hee, Cui Jian
Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China.
Phytother Res. 2009 Jul;23(7):938-42. doi: 10.1002/ptr.2834.
There is mounting evidence that peroxynitrite (ONOO(-)) is closely related to the pathogenesis of various diseases. As a pharmacological strategy aimed at preventing ONOO(-)-mediated toxicity, the protective activity of Forsythia suspensa (Thunb.) Vahl (Oleaceae) against ONOO(-)-induced cellular damage was investigated and its active components identified. After bioactivity-guided fractionation of its methylene chloride fraction, two tetrahydrofurofuran lignans were isolated, namely phillygenin and 8-hydroxypinoresinol. The protective effects of these lignans against ONOO(-)-induced cell death were evaluated using renal epithelial cell LLC-PK1. Phillygenin and 8-hydroxypinoresinol significantly reduced the cell injury by 3-morpholinosydnonimine (SIN-1), a ONOO(-) generator. The hydroxy substituents on the phenyl moieties may contribute to the antioxidant activities of these lignans. These results suggest that phillygenin and 8-hydroxypinoresinol may be useful for the therapeutic or preventive applications in treating ONOO(-)-related diseases.
越来越多的证据表明，过氧亚硝酸根（ONOO(-)）与多种疾病的发病机制密切相关。作为一种旨在预防ONOO(-)介导毒性的药理学策略，研究了连翘（木犀科）对ONOO(-)诱导的细胞损伤的保护活性，并鉴定了其活性成分。通过对其二氯甲烷馏分进行生物活性导向分离，分离出两种四氢呋喃呋喃木脂素，即连翘脂素和8-羟基松脂醇。使用肾上皮细胞LLC-PK1评估了这些木脂素对ONOO(-)诱导的细胞死亡的保护作用。连翘脂素和8-羟基松脂醇显著降低了由ONOO(-)发生器3-吗啉代辛二酮（SIN-1）诱导的细胞损伤。苯环上的羟基取代基可能有助于这些木脂素的抗氧化活性。这些结果表明，连翘脂素和8-羟基松脂醇可能在治疗或预防与ONOO(-)相关疾病中具有应用价值。
