Southam Andrew D, Easton John M, Stentiford Grant D, Ludwig Christian, Arvanitis Theodoros N, Viant Mark R
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
J Proteome Res. 2008 Dec;7(12):5277-85. doi: 10.1021/pr800353t.
Histopathologically well-characterized fish liver was analyzed by 800 MHz 1H NMR metabolomics to identify metabolic changes between healthy and tumor tissue. Data were analyzed by multivariate statistics and metabolic correlation networks, and results revealed elevated anaerobic metabolism and reduced choline metabolism in tumor tissue. Significant negative correlations were observed between alanine-acetate (p = 3.0 x 10(-5)) and between proline-acetate (p = 0.003) in tumors only, suggesting alanine and proline are utilized as alternative energy sources in flatfish liver tumors.
通过800兆赫的1H NMR代谢组学对组织病理学特征明确的鱼肝脏进行分析，以确定健康组织与肿瘤组织之间的代谢变化。采用多元统计和代谢相关网络对数据进行分析，结果显示肿瘤组织中无氧代谢升高，胆碱代谢降低。仅在肿瘤中观察到丙氨酸 - 乙酸盐之间（p = 3.0×10（-5））以及脯氨酸 - 乙酸盐之间（p = 0.003）存在显著负相关，这表明在比目鱼肝脏肿瘤中丙氨酸和脯氨酸被用作替代能源。
