Marks Vered, Munoz Anisleidys, Rai Priyamvada, Walls Jamie D
Department of Chemistry, University of Miami Coral Gables , FL , USA.
Department of Chemistry, University of MiamiCoral Gables, FL, USA; Department of Medicine, Division of Hematology and Oncology, University of Miami Miller School of Medicine, Miami, FL, USA.
PeerJ. 2016 Jun 7;4:e2104. doi: 10.7717/peerj.2104. eCollection 2016.
Metabolomic profiling is an increasingly important method for identifying potential biomarkers in cancer cells with a view towards improved diagnosis and treatment. Nuclear magnetic resonance (NMR) provides a potentially noninvasive means to accurately characterize differences in the metabolomic profiles of cells. In this work, we use (1)H NMR to measure the metabolomic profiles of water soluble metabolites extracted from isogenic control and oncogenic HRAS-, KRAS-, and NRAS-transduced BEAS2B lung epithelial cells to determine the robustness of NMR metabolomic profiling in detecting differences between the transformed cells and their untransformed counterparts as well as differences among the RAS-transformed cells. Unique metabolomic signatures between control and RAS-transformed cell lines as well as among the three RAS isoform-transformed lines were found by applying principal component analysis to the NMR data. This study provides a proof of principle demonstration that NMR-based metabolomic profiling can robustly distinguish untransformed and RAS-transformed cells as well as cells transformed with different RAS oncogenic isoforms. Thus, our data may potentially provide new diagnostic signatures for RAS-transformed cells.
代谢组学分析是一种日益重要的方法，旨在识别癌细胞中的潜在生物标志物，以改善癌症的诊断和治疗。核磁共振（NMR）提供了一种潜在的非侵入性手段，可准确表征细胞代谢组学图谱的差异。在这项工作中，我们使用氢核磁共振（¹H NMR）来测量从同基因对照细胞以及经致癌性HRAS、KRAS和NRAS转导的BEAS2B肺上皮细胞中提取的水溶性代谢物的代谢组学图谱，以确定NMR代谢组学分析在检测转化细胞与其未转化对应物之间的差异以及RAS转化细胞之间差异方面的稳健性。通过对NMR数据应用主成分分析，发现了对照细胞系与RAS转化细胞系之间以及三种RAS亚型转化细胞系之间独特的代谢组学特征。本研究提供了一个原理证明，即基于NMR的代谢组学分析能够可靠地区分未转化细胞和RAS转化细胞，以及用不同RAS致癌亚型转化的细胞。因此，我们的数据可能为RAS转化细胞提供新的诊断特征。
