Goodlad R A, Mandir N, Levin S, Allen J L, Wright N A
Histopathology Unit, Imperial Cancer Research Fund, London, England.
Gastroenterology. 1991 Nov;101(5):1229-34.
Colonic epithelial cell division, cell migration, and cell transit were investigated in dogs given 300 micrograms.kg-1.day-1 of the prostaglandin E1 analogue, misoprostol, for 11 weeks. The animals were then injected with [3H]thymidine and killed at timed intervals. The distribution of labeled and mitotic cells within the crypts was determined by scoring autoradiographs. There were no significant differences in mitotic index or labeling index between the two groups. The data were pooled and converted to give crypt cell production rates of 51.1 +/- 11.1 (control) and 58.24 +/- 8.6 cells per crypt per hour (test). However, the crypt length and cell population were slightly, but significantly, greater in the misoprostol-treated group (P less than 0.01). The movement of the wave of labeled cells with time after injection was used to calculate the median cell migration rates, which were 23.50 +/- 3.03 cell positions per day (control) and 18.30 +/- 2.56 (test). Thus, misoprostol had no significant effect on either the cell migration rate or the transit rates.
对给予前列腺素E1类似物米索前列醇300微克·千克-1·天-1共11周的犬进行结肠上皮细胞分裂、细胞迁移和细胞转运的研究。然后给动物注射[3H]胸腺嘧啶核苷，并在不同时间间隔处死。通过对放射自显影片评分来确定隐窝内标记细胞和有丝分裂细胞的分布。两组之间的有丝分裂指数或标记指数无显著差异。将数据汇总并换算后得出，隐窝细胞生成率为对照组每隐窝每小时51.1±11.1个细胞，试验组为每隐窝每小时58.24±8.6个细胞。然而，米索前列醇治疗组的隐窝长度和细胞数量略有增加，但差异显著（P<0.01）。利用注射后标记细胞波随时间的移动来计算中位细胞迁移率，分别为对照组每天23.50±3.03个细胞位置，试验组为18.30±2.56个细胞位置。因此，米索前列醇对细胞迁移率或转运率均无显著影响。
