Goodlad R A, Madgwick A J, Moffatt M R, Levin S, Allen J L, Wright N A
Imperial Cancer Research Fund, Histopathology Unit, London, England.
Gastroenterology. 1990 Jan;98(1):90-5. doi: 10.1016/0016-5085(90)91295-h.
Prostaglandins of the E series increase stomach mucosal mass by inducing hyperplasia, which could be the result either of increased cell production or of decreased cell loss. This report describes an investigation of the effect of the prostaglandin E1 analogue, misoprostol, on cell migration and transit. 3H-thymidine was used to label those cells synthesizing deoxyribonucleic acid in dogs that had been given an oral dose of 300 micrograms/kg per day misoprostol for 11 weeks. The animals were killed at timed intervals, and tissue from the gastric fundus was prepared for autoradiography. The distribution of labeled cells at various times after labeling was used to follow the movement of the wave of label and to calculate median cell migration rates and transit times. The migration rate of cells toward the gastric lumen was significantly increased from 1.4 +/- 0.3 to 3.6 +/- 0.6 cell positions per day in the misoprostol-treated group (p less than 0.001); however, the gland length (from the most basal mucous neck cell to the luminal surface) was also increased (from 52.1 +/- 1.1 to 74.0 +/- 1.6; p less than 0.001), thus there was no significant difference in the (transit) time taken for cells to reach the top of the gland (control, 17.5 +/- 9.8 days; test, 12.2 +/- 7.1 days).
E系列前列腺素通过诱导增生来增加胃黏膜质量，这可能是细胞产生增加或细胞损失减少的结果。本报告描述了对前列腺素E1类似物米索前列醇对细胞迁移和转运影响的研究。使用3H-胸腺嘧啶核苷标记给予每天300微克/千克米索前列醇口服剂量11周的犬体内合成脱氧核糖核酸的细胞。在不同时间点处死动物，并制备胃底组织用于放射自显影。标记后不同时间标记细胞的分布用于追踪标记波的移动，并计算细胞迁移率中位数和转运时间。在米索前列醇治疗组中，细胞向胃腔的迁移率从每天1.4±0.3个细胞位置显著增加到3.6±0.6个细胞位置（p<0.001）；然而，腺长度（从最基部的黏液颈细胞到腔表面）也增加了（从52.1±1.1增加到74.0±1.6；p<0.001），因此细胞到达腺顶部所需的（转运）时间没有显著差异（对照组，17.5±9.8天；试验组，12.2±7.1天）。
