Bertelsen Mads F, Kjelgaard-Hansen Mads, Grøndahl Carsten, Heegaard Peter M H, Jacobsen Stine
Center for Zoo and Wild Animal Health, Copenhagen Zoo, Roskildevej 38, DK-2000 Frederiksberg, Denmark.
J Zoo Wildl Med. 2009 Mar;40(1):199-203. doi: 10.1638/2007-0125.1.
The serum concentration of acute phase proteins (APPs) increases dramatically in response to inflammation and tissue injury. APPs are clinically useful in a range of domesticated mammals; however, knowledge is limited in nondomesticated mammals. The detective ability of two assays for each of three potential APPs--serum amyloid A (SAA), C-reactive protein (CRP), and haptoglobin (Hp)--was evaluated in eight species. For SAA, a turbidimetric immunoassay (TIA) demonstrated significant detective abilities in the Asian elephant (Elaphas maximus), impala (Aepyceros melampus), musk ox (Ovibos moschatus), and chimpanzee (Pan troglodytes), as did an SAA enzyme-linked immunosorbent assay (ELISA) in the impala. For CRP, both TIA and ELISA had significant detective abilities in the chimpanzee. For Hp, a colorimetric assay demonstrated significant detective abilities in impala, musk ox, sitatunga (Tragelaphus spekeii), and chimpanzee, as did the Hp ELISA in the impala, musk ox, and sitatunga. In conclusion, these results suggest that assays for detection of relevant APPs in several nondomesticated animals are available.
急性期蛋白（APPs）的血清浓度会因炎症和组织损伤而急剧增加。APPs在一系列家养哺乳动物中具有临床应用价值；然而，对于非家养哺乳动物的了解却很有限。对三种潜在的APPs——血清淀粉样蛋白A（SAA）、C反应蛋白（CRP）和触珠蛋白（Hp）——的两种检测方法在八个物种中的检测能力进行了评估。对于SAA，比浊免疫分析法（TIA）在亚洲象（印度象）、黑斑羚、麝牛和黑猩猩中显示出显著的检测能力，黑斑羚中的SAA酶联免疫吸附测定法（ELISA）也是如此。对于CRP，TIA和ELISA在黑猩猩中都具有显著的检测能力。对于Hp，比色法在黑斑羚、麝牛、水羚和黑猩猩中显示出显著的检测能力，黑斑羚、麝牛和水羚中的Hp ELISA也是如此。总之，这些结果表明有可用于检测几种非家养动物中相关APPs的检测方法。
