Zaichkina S I, Rozanova O M, Akhmadieva A Kh, Antikaeva G F, Sorokina S S, Smirnova E N, Romanchenko S P, Vakhrusheva O A
Radiats Biol Radioecol. 2009 Jan-Feb;49(1):55-9.
In present work, we investigated the genetic instability in mice of F1, of F2 and of F3 generations born from males irradiated by a low-dose rate of high-LET radiation that simulates the spectral and component composition of radiation fields formed in the conditions of high-altitude flights in vivo in polychromatic erythrocytes of bone marrow using the micronucleus test. Two-month-old males of SHK white mongrel mice were used. Irradiation was performed for 24 h a day in the radiation field behind the concrete shield of the U-70 accelerator of 70 GeV protons (Serpukhov) to accumulate doses of 11.5, of 21.5 and of 31.5 cGy (1 cGy/day). The experiments demonstrated that in mice of F1 generation born from males irradiated with doses of 11.5, 21.5 and of 31.5 cGy, an increase in sensitivity to additional irradiation with a dose of 1.5 Gy of gamma-radiation and the absence of adaptive response compared with the descendants of unirradiated males occur. In contrast to F1 generation genetic instability in mice of the F2 and F3 generations was revealed only by the absence of adaptive response. These data indicate a genetic instability in F1, F2 and F3 generations born from irradiated males.
在本研究中，我们利用微核试验，对低剂量率高传能线密度辐射照射的雄性小鼠所产F1、F2和F3代小鼠的遗传不稳定性进行了研究。这种辐射模拟了高空飞行条件下体内形成的辐射场的光谱和成分组成，实验对象为骨髓多色红细胞。使用了2月龄的SHK白色杂种雄性小鼠。在70 GeV质子的U - 70加速器（谢尔普霍夫）混凝土屏蔽后的辐射场中，每天照射24小时，以累积11.5、21.5和31.5 cGy的剂量（1 cGy/天）。实验表明，由接受11.5、21.5和31.5 cGy剂量照射的雄性小鼠所产F1代小鼠，与未受照射雄性小鼠的后代相比，对1.5 Gyγ射线的额外照射敏感性增加，且不存在适应性反应。与F1代不同，F2和F3代小鼠的遗传不稳定性仅表现为不存在适应性反应。这些数据表明，受照射雄性小鼠所产F1、F2和F3代存在遗传不稳定性。
