Mirkovitch V, Campiche M
Eur Surg Res. 1977;9(3):173-90. doi: 10.1159/000127937.
In mongrel dogs, the horizontal part of the pancreas was infiltrated with collagenase, cut in pieces, incubated with collagenase, rinsed twice by centrifugation or sedimentation, and implanted into the spleen of the same animal. The operations were terminated by the removal of the rest of the pancreas. Of 26 operated dogs, one died because of a duodenal perforation, five developed severe hyperglycaemia without remission, and 20 were long-term normoglycaemic survivors followed for up to 10 weeks. These 20 animals became spontaneously normoglycaemic in the course of the first 10 postoperative days. Later, during glucose loading tests, the pattern of blood sugar values was the same in the transplanted animals as in those of a group of non-operated dogs, but the insulin release, although immediate, attained half the control values. The plasma insulin in the splenic vein was more than seven times higher than in the peripheral circulation. Splenectomies performed in seven animals were followed by severe hyperglycaemia and death. Light and electron microscopy demonstrated the presence of the intact endocrine and exocrine pancreatic tissues in the spleens of all animals investigated. It is concluded that laborious separations of endocrine from exocrine tissue are not mandatory for ulterior endocrine function, and that in an animal larger than rodents it is possible to obtain a diabetes-preventing function after the transplantation of only a part of the gland.
在杂种狗身上，用胶原酶浸润胰腺的水平部分，切成小块，用胶原酶孵育，通过离心或沉降冲洗两次，然后植入同一动物的脾脏。手术通过切除剩余的胰腺来结束。在26只接受手术的狗中，1只因十二指肠穿孔死亡，5只出现严重高血糖且未缓解，20只成为长期血糖正常的存活者，随访长达10周。这20只动物在术后的头10天内自发地血糖恢复正常。后来，在葡萄糖负荷试验中，移植动物的血糖值模式与一组未手术的狗相同，但胰岛素释放虽然迅速，但仅达到对照值的一半。脾静脉中的血浆胰岛素比外周循环中的高七倍多。对7只动物进行脾切除术后，出现严重高血糖并死亡。光镜和电镜检查显示，在所有研究动物的脾脏中均存在完整的胰腺内分泌和外分泌组织。结论是，为了后续的内分泌功能，费力地将内分泌组织与外分泌组织分离并非必要，并且在比啮齿动物更大的动物中，仅移植部分腺体后就有可能获得预防糖尿病的功能。
