Oshio Ichiro, Osaki Takako, Hanawa Tomoko, Yonezawa Hideo, Zaman Cynthia, Kurata Satoshi, Kamiya Shigeru
Group of Metabolism and Chemistry, Department of Environmental Science and Toxicology, Odawara Research Center, Nippon Soda Co. Ltd, Tokyo, Japan.
Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Japan.
J Med Microbiol. 2009 May;58(Pt 5):656-662. doi: 10.1099/jmm.0.008185-0.
To identify the time frame and route of mother-to-child Helicobacter pylori infection, a Mongolian gerbil model was used. Four-week-old female Mongolian gerbils were infected with H. pylori, and then mated with uninfected males 2 months after infection. The offspring were sacrificed weekly after birth, and then serum, mother's milk from the stomach and gastric tissues were obtained from pups. Anti-H. pylori antibody titres were measured in sera and maternal milk using an ELISA. The stomach was cut in two in the sagittal plane, and then H. pylori colonization in mucosa was confirmed by culture and real-time RT-PCR in one specimen and by immunochemical staining in the other. Faeces and oral swabs were obtained from infected mothers, and H. pylori 16S rRNA was measured using real-time RT-PCR. H. pylori was not identified in cultures from the gastric mucosa of pups delivered by infected mothers, but H. pylori 16S rRNA was detected from 4 weeks after birth, suggesting that Mongolian gerbil pups become infected via maternal H. pylori transmission from 4 weeks of age. The anti-H. pylori antibody titre in sera of pups from infected mothers was maximum at 3 weeks of age and then rapidly decreased from 4 weeks of age. High antibody titres in mother's milk were detected during the suckling period, and GlcNAcalpha was detectable at 2-4 weeks of age, but disappeared as the offspring aged. Thus H. pylori seems to infect Mongolian gerbil pups from 4 weeks of age, in parallel with decreasing GlcNAcalpha expression in the gastric mucosa. These results suggested that H. pylori infection of Mongolian gerbil pups occurs via faecal-oral transmission from an infected mother.
为了确定母婴间幽门螺杆菌感染的时间框架和途径，使用了蒙古沙鼠模型。4周龄的雌性蒙古沙鼠感染幽门螺杆菌，感染2个月后与未感染的雄性交配。后代出生后每周处死，然后从幼崽中获取血清、胃内的母乳和胃组织。使用酶联免疫吸附测定法（ELISA）测量血清和母乳中的抗幽门螺杆菌抗体滴度。将胃沿矢状面切成两半，然后在一个标本中通过培养和实时逆转录聚合酶链反应（RT-PCR）以及在另一个标本中通过免疫化学染色确认黏膜中的幽门螺杆菌定植。从感染的母亲处获取粪便和口腔拭子，使用实时RT-PCR测量幽门螺杆菌16S rRNA。在感染母亲所生幼崽的胃黏膜培养物中未鉴定出幽门螺杆菌，但从出生后4周开始检测到幽门螺杆菌16S rRNA，这表明蒙古沙鼠幼崽从4周龄开始通过母体幽门螺杆菌传播而被感染。感染母亲所生幼崽血清中的抗幽门螺杆菌抗体滴度在3周龄时最高，然后从4周龄开始迅速下降。在哺乳期检测到母乳中的抗体滴度较高，并且在2至4周龄时可检测到N-乙酰葡糖胺（GlcNAcalpha），但随着后代年龄增长而消失。因此，幽门螺杆菌似乎从4周龄开始感染蒙古沙鼠幼崽，同时胃黏膜中GlcNAcalpha的表达下降。这些结果表明，蒙古沙鼠幼崽的幽门螺杆菌感染是通过受感染母亲的粪-口传播发生的。
