Lee Jin-Uk, Kim Okjin
Animal Disease Research Unit, College of Life Science and Natural Resources, Wonkwang University, Iksan 570-749, South Korea.
World J Gastroenterol. 2006 Sep 21;12(35):5663-7. doi: 10.3748/wjg.v12.i35.5663.
To investigate maternal H. pylori infection status to determine the potential of maternal transmission.
In the present study, we examined these issues in an experimental murine model, which is a Mongolian gerbil model that has been reported as an optimal laboratory animal model to study H. pylori. Pregnant Mongolian gerbils, infected experimentally with H. pylori, were divided into as four groups. Following the experimental design, the stomachs of the mother and litters were isolated and assessed for transmission of H. pylori at the prenatal period, parturition day, 1-wk old and 3-wk old respectively. Bacterial culture and polymerase chain reaction (PCR) were used to examine the presence of transmitted H. pylori.
All litters showed no transmission of H. pylori during pregnancy and at parturition day. However, they revealed 33.3% and 69.6% at 1-wk and 3-wk of age respectively by PCR.
These results suggested that vertical infection during the prenatal period or delivery procedure is unlikely as a route of mother-to-child H pylori infection. It may be that H. pylori is acquired through breast-feeding, contaminated saliva and fecal-oral transmission during co-habitation.
调查母亲幽门螺杆菌感染状况，以确定母婴传播的可能性。
在本研究中，我们在一种实验性小鼠模型中研究了这些问题，该模型是蒙古沙鼠模型，已被报道为研究幽门螺杆菌的最佳实验动物模型。将经实验感染幽门螺杆菌的怀孕蒙古沙鼠分为四组。按照实验设计，分别在产前、分娩日、1周龄和3周龄时分离母鼠及其幼崽的胃，并评估幽门螺杆菌的传播情况。采用细菌培养和聚合酶链反应（PCR）检测传播的幽门螺杆菌的存在情况。
所有幼崽在孕期和分娩日均未出现幽门螺杆菌传播。然而，通过PCR检测发现，它们在1周龄和3周龄时幽门螺杆菌传播率分别为33.3%和69.6%。
这些结果表明，产前或分娩过程中的垂直感染不太可能是母婴幽门螺杆菌感染的途径。幽门螺杆菌可能是通过母乳喂养、受污染的唾液以及同居期间的粪口传播而获得的。
