Monge Claire, Beraud Nathalie, Tepp Kersti, Pelloux Sophie, Chahboun Siham, Kaambre Tuuli, Kadaja Lumme, Roosimaa Mart, Piirsoo Andres, Tourneur Yves, Kuznetsov Andrey V, Saks Valdur, Seppet Enn
INSERM U884, Laboratory of Fundamental and Applied Bioenergetics, Joseph Fourier University, Grenoble, France.
Can J Physiol Pharmacol. 2009 Apr;87(4):318-26. doi: 10.1139/Y09-018.
Comparative analysis of the bioenergetic parameters of adult rat cardiomyocytes (CM) and HL-1 cells with very different structure but similar cardiac phenotype was carried out with the aim of revealing the importance of the cell structure for regulation of its energy fluxes. Confocal microscopic analysis showed very different mitochondrial arrangement in these cells. The cytochrome content per milligram of cell protein was decreased in HL-1 cells by a factor of 7 compared with CM. In parallel, the respiratory chain complex activities were decreased by 4-8 times in the HL-1 cells. On the contrary, the activities of glycolytic enzymes, hexokinase (HK), and pyruvate kinase (PK) were increased in HL-1 cells, and these cells effectively transformed glucose into lactate. At the same time, the creatine kinase (CK) activity was significantly decreased in HL-1 cells. In conclusion, the results of this study comply with the assumption that in contrast to CM in which oxidative phosphorylation is a predominant provider of ATP and the CK system is a main carrier of energy from mitochondria to ATPases, in HL-1 cells the energy metabolism is based mostly on the glycolytic reactions coupled to oxidative phosphorylation through HK.
对结构迥异但心脏表型相似的成年大鼠心肌细胞（CM）和HL-1细胞的生物能量参数进行了比较分析，目的是揭示细胞结构对其能量通量调节的重要性。共聚焦显微镜分析显示这些细胞中线粒体排列差异很大。与CM相比，HL-1细胞中每毫克细胞蛋白的细胞色素含量降低了7倍。同时，HL-1细胞中呼吸链复合物的活性降低了4至8倍。相反，HL-1细胞中糖酵解酶、己糖激酶（HK）和丙酮酸激酶（PK）的活性增加，并且这些细胞有效地将葡萄糖转化为乳酸。与此同时，HL-1细胞中的肌酸激酶（CK）活性显著降低。总之，本研究结果符合以下假设：与CM中氧化磷酸化是ATP的主要供应者且CK系统是能量从线粒体到ATP酶的主要载体不同，HL-1细胞中的能量代谢主要基于通过HK与氧化磷酸化偶联的糖酵解反应。
