Hirochika H, Hayashi K
Department of Molecular Biology, National Institute of Agrobiological Resources, Ibaraki, Japan.
Gene. 1991 Sep 15;105(2):239-41. doi: 10.1016/0378-1119(91)90156-6.
Co-infection of plants with non-overlapping deletion mutants of cauliflower mosaic virus usually leads to the production of the wild-type virus. To prevent this, a pair of mutants with overlapping deletions was constructed. In infected plants both mutant DNAs were stably maintained. Such mutants with overlapping deletions will be used as a vector to overcome the size limitation of genes to be cloned.
用花椰菜花叶病毒的非重叠缺失突变体对植物进行共感染通常会导致野生型病毒的产生。为防止这种情况，构建了一对具有重叠缺失的突变体。在受感染的植物中，两种突变体DNA都能稳定维持。这种具有重叠缺失的突变体将被用作载体，以克服待克隆基因的大小限制。
