Cooksey Gregory A, Plant Anne L, Atencia Javier
Biochemical Science Division, NIST, Gaithersburg, MD, USA.
Lab Chip. 2009 May 7;9(9):1298-300. doi: 10.1039/b820683j. Epub 2009 Feb 17.
The lack of simple interfaces for microfluidic devices with a large number of inlets significantly limits production and utilization of these devices. In this article, we describe the fabrication of a reusable manifold that provides rapid world-to-chip connectivity. A vacuum network milled into a rigid manifold holds microdevices and prevents leakage of fluids injected into the device from ports in the manifold. A number of different manifold designs were explored, and all performed similarly, yielding an average of 100 kPa (15 psi) fluid holding pressure. The wide applicability of this manifold concept is demonstrated by interfacing with a 51-inlet microfluidic chip containing 144 chambers and hundreds of embedded pneumatic valves. Due to the speed of connectivity, the manifolds are ideal for rapid prototyping and are well suited to serve as "universal" interfaces.
具有大量入口的微流控设备缺乏简单的接口，这严重限制了这些设备的生产和应用。在本文中，我们描述了一种可重复使用的歧管的制造方法，该歧管可实现快速的从外界到芯片的连接。铣削到刚性歧管中的真空网络可固定微型设备，并防止从歧管端口注入设备的流体泄漏。我们探索了多种不同的歧管设计，所有设计的性能都相似，产生的流体保持压力平均为100 kPa（15 psi）。通过与一个包含144个腔室和数百个嵌入式气动阀的51入口微流控芯片连接，证明了这种歧管概念的广泛适用性。由于连接速度快，这些歧管非常适合快速原型制作，并且非常适合用作“通用”接口。
