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Phage T4 expression vector: protection from proteolysis.

作者信息

Singer B S, Gold L

机构信息

Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309.

出版信息

Gene. 1991 Sep 30;106(1):1-6. doi: 10.1016/0378-1119(91)90558-s.

DOI:10.1016/0378-1119(91)90558-s
PMID:1937029
Abstract

We have developed an efficient method for the expression of heterologous genes during infection by T4, a bacteriophage known to inhibit the proteolytic systems of Escherichia coli. This system enables us to clone genes in a plasmid expression vector and move them readily into T4. We have used lacZ as a reporter gene to show that both plasmid and phage exhibit low basal expression or high-level expression under the control of a T7 promoter. This system promises a possible solution to the problem of degradation and/or toxicity of overproduced proteins.

摘要

相似文献

1
Phage T4 expression vector: protection from proteolysis.
Gene. 1991 Sep 30;106(1):1-6. doi: 10.1016/0378-1119(91)90558-s.
2
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[Cloning of the DNA BglII fragments of bacteriophage T4 in the vector plasmid pSCC31].[噬菌体T4的DNA BglII片段在载体质粒pSCC31中的克隆]
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Regulation of transcription in the segment of the bacteriophage T4 genome containing three of the head protein genes: plasmid-specific and phage chromosome-specific effects.噬菌体T4基因组中包含三个头部蛋白基因的片段的转录调控：质粒特异性和噬菌体染色体特异性效应。
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