Vanhanen S, Saloheimo A, Ilmén M, Knowles J K, Penttilä M
VTT Biotechnical Laboratory, Espoo, Finland.
Gene. 1991 Sep 30;106(1):129-33. doi: 10.1016/0378-1119(91)90577-x.
Transcription of the 3-phosphoglycerate kinase (PGK)-encoding gene (pgk1) of Trichoderma reesei results in two transcripts due to two main transcription start points (tsp) which are differentially regulated during the growth cycle. The nucleotide sequence of the promoter reveals a number of putative regulatory elements present also in the PGK promoter of Saccharomyces cerevisiae: a 20-nt long sequence similar to the CTTCC-repeat region of the upstream activating sequence UAS, the eukaryotic heat-shock consensus sequence, HSE, and a putative eukaryotic cAMP regulatory sequence. The functionality of the putative HSE sequence was examined, but no clear effect could be seen on the total amount of pgk1 mRNA at elevated temperatures nor on transcription initiation from the upstream tsp, preceded by the HSE sequence.
里氏木霉3-磷酸甘油酸激酶（PGK）编码基因（pgk1）的转录产生两种转录本，这是由于两个主要转录起始点（tsp）所致，它们在生长周期中受到不同的调控。启动子的核苷酸序列揭示了一些也存在于酿酒酵母PGK启动子中的假定调控元件：一段20个核苷酸长的序列，类似于上游激活序列UAS的CTTCC重复区域、真核热休克共有序列HSE以及一个假定的真核cAMP调控序列。对假定的HSE序列的功能进行了检测，但在高温下对pgk1 mRNA的总量以及在HSE序列之前的上游tsp的转录起始均未观察到明显影响。
