Wei Gang, Tao Yong, Liu Guozhen, Chen Chen, Luo Renyuan, Xia Hongai, Gan Qiang, Zeng Haipan, Lu Zhike, Han Yuning, Li Xiaobing, Song Guisheng, Zhai Hongli, Peng Yonggang, Li Dayong, Xu Honglin, Wei Xiaoli, Cao Mengliang, Deng Huafeng, Xin Yeyun, Fu Xiqin, Yuan Longping, Yu Jun, Zhu Zhen, Zhu Lihuang
State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Proc Natl Acad Sci U S A. 2009 May 12;106(19):7695-701. doi: 10.1073/pnas.0902340106. Epub 2009 Apr 16.
By using a whole-genome oligonucleotide microarray, designed based on known and predicted indica rice genes, we investigated transcriptome profiles in developing leaves and panicles of superhybrid rice LYP9 and its parental cultivars 93-11 and PA64s. We detected 22,266 expressed genes out of 36,926 total genes set collectively from 7 tissues, including leaves at seedling and tillering stages, flag leaves at booting, heading, flowering, and filling stages, and panicles at filling stage. Clustering results showed that the F1 hybrid's expression profiles resembled those of its parental lines more than that which lies between the 2 parental lines. Out of the total gene set, 7,078 genes are shared by all sampled tissues and 3,926 genes (10.6% of the total gene set) are differentially expressed genes (DG). As we divided DG into those between the parents (DG(PP)) and between the hybrid and its parents (DG(HP)), the comparative results showed that genes in the categories of energy metabolism and transport are enriched in DG(HP) rather than in DG(PP). In addition, we correlated the concurrence of DG and yield-related quantitative trait loci, providing a potential group of heterosis-related genes.
通过使用基于已知和预测的籼稻基因设计的全基因组寡核苷酸微阵列，我们研究了超级杂交稻LYP9及其亲本品种93 - 11和PA64s在发育中的叶片和穗中的转录组图谱。我们从7个组织（包括苗期和分蘖期的叶片、孕穗期、抽穗期、开花期和灌浆期的剑叶以及灌浆期的穗）共同设置的36926个总基因中检测到22266个表达基因。聚类结果表明，F1杂种的表达谱与其亲本系的表达谱更相似，而不是介于两个亲本系之间。在整个基因集中，7078个基因在所有采样组织中都有共享，3926个基因（占总基因集的10.6%）是差异表达基因（DG）。当我们将DG分为亲本之间的（DG(PP)）和杂种与其亲本之间的（DG(HP)）时，比较结果表明，能量代谢和转运类别中的基因在DG(HP)中富集，而不是在DG(PP)中。此外，我们将DG与产量相关的数量性状位点的并发情况进行了关联，提供了一组潜在的杂种优势相关基因。
