亚种间杂交稻耐盐杂种优势的比较转录组分析。
Comparative Transcriptome Analysis of the Heterosis of Salt Tolerance in Inter-Subspecific Hybrid Rice.
机构信息
Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
The Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China.
出版信息
Int J Mol Sci. 2023 Jan 22;24(3):2212. doi: 10.3390/ijms24032212.
Soil salinity is one of the major abiotic stresses limiting rice growth. Hybrids outperform their parents in salt tolerance in rice, while its mechanism is not completely understood. In this study, a higher seedling survival was observed after salt treatment in an inter-subspecific hybrid rice, Zhegengyou1578 (ZGY1578), compared with its maternal Zhegeng7A (ZG7A) and paternal Zhehui1578 (ZH1578). A total of 2584 and 3061 differentially expressed genes (DEGs) with at least twofold changes were identified between ZGY1578 and ZG7A and between ZGY1578 and ZH1578, respectively, in roots under salt stress using the RNA sequencing (RNA-Seq) approach. The expressions of a larger number of DEGs in hybrid were lower or higher than those of both parents. The DEGs associated with transcription factors, hormones, and reactive oxygen species (ROS)-related genes might be involved in the heterosis of salt tolerance. The expressions of the majority of transcription factors and ethylene-, auxin-, and gibberellin-related genes, as well as peroxidase genes, were significantly higher in the hybrid ZGY1578 compared with those of both parents. The identified genes provide valuable clues to elucidate the heterosis of salt tolerance in inter-subspecific hybrid rice.
土壤盐度是限制水稻生长的主要非生物胁迫之一。在水稻中，杂种在耐盐性方面优于其双亲，但其机制尚不完全清楚。在本研究中，与母本浙梗 7A（ZG7A）和父本浙恢 1578（ZH1578）相比，亚种间杂交稻浙梗优 1578（ZGY1578）在盐处理后幼苗存活率更高。利用 RNA 测序（RNA-Seq）方法，分别在根中鉴定到 ZGY1578 与 ZG7A 和 ZGY1578 与 ZH1578 之间至少有两倍变化的 2584 个和 3061 个差异表达基因（DEGs）。杂种中更多的 DEGs 的表达低于或高于双亲。与转录因子、激素和活性氧（ROS）相关基因相关的 DEGs 可能参与了耐盐杂种优势。大多数转录因子和乙烯、生长素和赤霉素相关基因以及过氧化物酶基因的表达在杂种 ZGY1578 中明显高于双亲。所鉴定的基因为阐明亚种间杂交稻耐盐杂种优势提供了有价值的线索。
Zotero 插件