XC DNA 的剪切对其劳斯肉瘤病毒转染活性的影响。
The effect of shearing of XC DNA on its RSV transfecting activity.
作者信息
Svoboda J, Zadrazil S
出版信息
Folia Biol (Praha). 1977;23(2):125-9.
PMID:193741
Abstract
XC DNA fragments having the average molecular weight of 10(7) daltons were obtained by hydrodynamic shearing followed by sucrose gradient centrifugation or BUdR treatment of XC cells used for DNA preparation. Such DNA molecules, even after long-term storage, were found to be active in transfection.
摘要
通过流体动力学剪切，随后进行蔗糖梯度离心或对用于制备DNA的XC细胞进行溴脱氧尿苷（BUdR）处理，获得了平均分子量为10⁷道尔顿的XC DNA片段。发现即使经过长期储存，此类DNA分子在转染中仍具有活性。
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