Elmoazzen Heidi Y, Lee Gloria Y, Li Ming W, McGinnis Lynda K, Lloyd K C Kent, Toner Mehmet, Biggers John D
Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, Boston, MA 02115, USA.
Cryobiology. 2009 Aug;59(1):113-5. doi: 10.1016/j.cryobiol.2009.03.005. Epub 2009 Apr 16.
It has been shown in the past that mouse spermatozoa could be dried under a stream of nitrogen gas at ambient temperature and stored at 4 degrees C or 22 degrees C for up to 3 months and was capable of generating live-born offspring. In previous desiccation work, dried sperm were stored in a vacuum-sealed plastic bag placed in a vacuum-packed Mylar bag. However, dried specimens stored in this way often lost moisture, particularly in samples stored at higher temperatures (22 degrees C) compared to lower temperatures (4 degrees C). The present report describes a method which minimizes this water loss from the dried sperm samples. Its use is described in a preliminary study on the effect of supplementing the trehalose with glycerol. The results have demonstrated that mouse sperm can be stored at 4 degrees C over saturated NaBr without the uptake of water which occurs when they are stored in Mylar packages. In addition, we were able to get some survival of sperm (9-15%) at room temperature storage after 3 months. The addition of glycerol to trehalose had little effect on the survival of dried mouse sperm stored over NaBr for 1 and 3 months.
过去的研究表明，小鼠精子可以在室温下于氮气气流中干燥，并在4℃或22℃下储存长达3个月，且能够产生活产后代。在之前的干燥研究中，干燥后的精子被储存在置于真空包装的聚酯薄膜袋中的真空密封塑料袋中。然而，以这种方式储存的干燥标本经常会失去水分，尤其是与低温（4℃）相比，在高温（22℃）下储存的样本。本报告描述了一种将干燥精子样本的水分流失降至最低的方法。其用途在一项关于用甘油补充海藻糖效果的初步研究中有所描述。结果表明，小鼠精子可以在4℃下储存在饱和溴化钠上方，而不会像储存在聚酯薄膜包装中那样吸收水分。此外，我们能够在室温下储存3个月后使部分精子存活（9 - 15%）。在海藻糖中添加甘油对在溴化钠上方储存1个月和3个月的干燥小鼠精子的存活率影响不大。
