Liu Jie, Lee Gloria Y, Lawitts Joel A, Toner Mehmet, Biggers John D
Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, Boston, Massachusetts, United States of America.
Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States of America.
PLoS One. 2014 Jun 12;9(6):e99809. doi: 10.1371/journal.pone.0099809. eCollection 2014.
The purpose of this study is to develop a mouse sperm preservation method based on evaporative drying. Mouse sperm were evaporatively dried and stored at 4°C and ambient temperature for 3 months to 2 years. Upon rehydration, a single sperm was injected into a mature oocyte to develop into a blastocyst after culture or a live birth after embryo transfer to a recipient female. For the samples stored at 4°C for 3, 6, 12, 18, and 24 months, the blastocyst formation rate was 61.5%, 49.1%, 31.5%, 32.2%, and 41.4%, respectively. The blastocyst rate for those stored at ambient temperature (∼22°C) for 3, 6, 12, and 18 months was 57.8%, 36.2%, 33.6%, and 34.4%, respectively. Fifteen, eight and three live pups were produced from sperm stored at room temperature for 12, 18, and 24 months, respectively. This is the first report of live offspring produced from dried mouse sperm stored at ambient temperature for up to 2 years. Based on these results, we suggest that evaporative drying is a potentially useful method for the routine preservation of mouse sperm.
本研究的目的是开发一种基于蒸发干燥的小鼠精子保存方法。将小鼠精子进行蒸发干燥，并在4°C和室温下储存3个月至2年。复水后，将单个精子注射到成熟卵母细胞中，经培养发育成囊胚，或移植到受体雌性动物体内后产下活体幼崽。对于在4°C下储存3、6、12、18和24个月的样本，囊胚形成率分别为61.5%、49.1%、31.5%、32.2%和41.4%。在室温（约22°C）下储存3、6、12和18个月的样本，其囊胚率分别为57.8%、36.2%、33.6%和34.4%。分别从在室温下储存12、18和24个月的精子中产出了15只、8只和3只活体幼崽。这是关于在室温下干燥保存长达2年的小鼠精子产生活体后代的首次报道。基于这些结果，我们认为蒸发干燥是一种用于小鼠精子常规保存的潜在有用方法。
