Nicolás Francisco E, Torres-Martínez Santiago, Ruiz-Vázquez Rosa M
Departamento de Genética y Microbiología (Unidad Asociada al IQFR-CSIC), Facultad de Biología, Universidad de Murcia, 30071 Murcia, Spain.
J Biotechnol. 2009 Jun 15;142(2):123-6. doi: 10.1016/j.jbiotec.2009.04.003. Epub 2009 Apr 16.
Initiation and maintenance of the RNA silencing mechanism was investigated after transcriptional activation of the transgene carB in Mucor circinelloides. Light induced transcription of the reporter gene carB specifically increased the 21-nt siRNA compared with the 25-nt siRNA and rose the silencing maintenance from 48% up to 93% of the descendent colonies. In accordance, induced transcription of the gene carB through disruption of the gene crgA increased the initial silencing frequency up to 30-fold, when compared with the frequency of silencing obtained using a crgA(+) genetic background.
在卷枝毛霉中转基因carB转录激活后，对RNA沉默机制的启动和维持进行了研究。与25 nt的小干扰RNA（siRNA）相比，光诱导报告基因carB的转录特异性增加了21 nt的siRNA，并将沉默维持率从后代菌落的48%提高到93%。相应地，通过破坏基因crgA诱导carB基因的转录，与使用crgA(+)遗传背景获得的沉默频率相比，初始沉默频率提高了30倍。
