Jung Mi, Oh Byung-Keun, Choi Jeong-Woo
Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 121-742, Republic of Korea.
Ultramicroscopy. 2009 Jul;109(8):1006-10. doi: 10.1016/j.ultramic.2009.03.045. Epub 2009 Mar 28.
Au nanodots can be utilized for the fabrication of protein nanoarrays. Au nanodots were fabricated on ITO glass by the thermal evaporation method using nanoporous alumina as a shadow mask. Uniform Au nanodots with a diameter of 60nm were formed on the ITO glass as a replica of the alumina mask. The morphology of the Au nanodots was verified by Field Emission Scanning Electron Microscopy (FE-SEM). Cysteine-modified azurins were immobilized on the Au nanodots. The topography of the proteins immobilized on the Au nanodots was investigated by atomic force microscopy (AFM) in tapping mode. These Au dot arrays can be potentially utilized as usable elements to construct nanobiochips.
金纳米点可用于制造蛋白质纳米阵列。通过使用纳米多孔氧化铝作为荫罩的热蒸发法在ITO玻璃上制备金纳米点。在ITO玻璃上形成了直径为60nm的均匀金纳米点，作为氧化铝掩膜的复制品。通过场发射扫描电子显微镜（FE-SEM）验证了金纳米点的形态。将半胱氨酸修饰的天青蛋白固定在金纳米点上。通过原子力显微镜（AFM）的轻敲模式研究固定在金纳米点上的蛋白质的形貌。这些金点阵列有可能用作构建纳米生物芯片的可用元件。
