Department of Interdisciplinary program of Integrated Biotechnology, Sogang University, Seoul, Korea.
Ultramicroscopy. 2010 May;110(6):676-81. doi: 10.1016/j.ultramic.2010.02.031. Epub 2010 Feb 24.
In this study, in situ electrochemical synthesis of polypyrrole nanowires with nanoporous alumina template was described. The formation of highly ordered porous alumina substrate was demonstrated with Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM). In addition, Fourier transform infrared analysis confirmed that polypyrrole (PP) nanowires were synthesized by direct electrochemical oxidation of pyrrole. HeLa cancer cells and HMCF normal cells were immobilized on the polypyrrole nanowires/nanoporous alumina substrates to determine the effects of the substrate on the cell morphology, adhesion and proliferation as well as the biocompatibility of the substrate. Cell adhesion and proliferation were characterized using a standard MTT assay. The effects of the polypyrrole nanowires/nanoporous alumina substrate on the cell morphology were studied by AFM. The nanoporous alumina coated with polypyrrole nanowires was found to exhibit better cell adhesion and proliferation than polystyrene petridish, aluminum foil, 1st anodized and uncoated 2nd anodized alumina substrate. This study showed the potential of the polypyrrole nanowires/nanoporous alumina substrate as biocompatibility electroactive polymer substrate for both healthy and cancer cell cultures applications.
本研究描述了一种以纳米多孔氧化铝模板原位电化学合成聚吡咯纳米线的方法。原子力显微镜（AFM）和扫描电子显微镜（SEM）的结果证实了高度有序的多孔氧化铝基底的形成。此外，傅里叶变换红外分析证实聚吡咯（PP）纳米线是通过吡咯的直接电化学氧化合成的。将 HeLa 癌细胞和 HMCF 正常细胞固定在聚吡咯纳米线/纳米多孔氧化铝基底上，以确定基底对细胞形态、黏附和增殖以及基底的生物相容性的影响。细胞黏附和增殖通过标准 MTT 测定进行了表征。通过原子力显微镜研究了聚吡咯纳米线/纳米多孔氧化铝基底对细胞形态的影响。结果表明，涂覆有聚吡咯纳米线的纳米多孔氧化铝比聚苯乙烯培养皿、铝箔、第一次阳极氧化和未涂覆的第二次阳极氧化铝基底具有更好的细胞黏附和增殖能力。本研究表明，聚吡咯纳米线/纳米多孔氧化铝基底作为生物相容性的电活性聚合物基底，具有应用于健康细胞和癌细胞培养的潜力。
