Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, Korea.
Nanotechnology. 2011 Jun 10;22(23):235304. doi: 10.1088/0957-4484/22/23/235304. Epub 2011 Apr 11.
Two-dimensional gold (Au) nanodot arrays on a transparent substrate were fabricated for imaging of living cells. A nanoporous alumina mask with large-area coverage capability was prepared by a two-step chemical wet etching process after a second anodization. Highly ordered Au nanodot arrays were formed on indium-tin-oxide (ITO) glass using very thin nanoporous alumina of approximately 200 nm thickness as an evaporation mask. The large-area Au nanodot arrays on ITO glass were modified with RGD peptide (arginine; glycine; aspartic acid) containing a cysteine (Cys) residue and then used to immobilize human cancer HeLa cells, the morphology of which was observed by confocal microscopy. The confocal micrographs of living HeLa cells on Au nanodot arrays revealed enhanced contrast and resolution, which enabled discernment of cytoplasmic organelles more clearly. These results suggest that two-dimensional Au nanodot arrays modified with RGD peptide on ITO glass have potential as a biocompatible nanobioplatform for the label-free visualization and adhesion of living cells.
在透明衬底上制备了二维金（Au）纳米点阵列，用于活细胞成像。通过两步化学湿法刻蚀工艺，在二次阳极氧化后制备了具有大面积覆盖能力的纳米多孔氧化铝掩模。使用约 200nm 厚的非常薄的纳米多孔氧化铝作为蒸发掩模，在铟锡氧化物（ITO）玻璃上形成了高度有序的 Au 纳米点阵列。将含有半胱氨酸（Cys）残基的精氨酸（Arg）；甘氨酸（Gly）；天冬氨酸（Asp）的 RGD 肽修饰大面积 ITO 玻璃上的 Au 纳米点阵列，然后用于固定人癌细胞 HeLa，通过共聚焦显微镜观察其形态。活 HeLa 细胞在 Au 纳米点阵列上的共聚焦显微镜图像显示出增强的对比度和分辨率，能够更清晰地区分细胞质细胞器。这些结果表明，ITO 玻璃上用 RGD 肽修饰的二维 Au 纳米点阵列具有作为无标记可视化和活细胞粘附的生物相容性纳米生物平台的潜力。
