Baveja U K, Roy T, Kaur M, Kulpati D D, Aggarwal S K
Department of Microbiology, Maulana Azad Medical College & Associated Hospitals, New Delhi.
Indian J Med Res. 1991 May;93:161-5.
Sera from 34 patients of amoebic liver abscess (ALA) and 11 patients with amoebic dysentery (AD) were examined for the presence of specific Entamoeba histolytica (EH) antibodies and amoebic antigen by enzyme linked immunosorbent assay (ELISA) and dot immunobinding assay (DIB). Both techniques were found to be equally sensitive for detecting antiamoebic antibodies (89.5 and 91.9% respectively) and highly specific (100%) in patients of ALA. ELISA was found to be more sensitive (94.4%) in detecting circulating amoebic antigen compared to DIB (68%) in patients of ALA. Specific antibodies, in significant levels, were detected in 3 and 5 patients of AD by ELISA and DIB assay, respectively. As DIB assay is easier to perform and less expensive, is recommended for detection of antibodies in patients with invasive amoebiasis.
采用酶联免疫吸附测定（ELISA）和斑点免疫结合测定（DIB）检测了34例阿米巴肝脓肿（ALA）患者和11例阿米巴痢疾（AD）患者血清中特异性溶组织内阿米巴（EH）抗体及阿米巴抗原的存在情况。结果发现，这两种技术在检测ALA患者抗阿米巴抗体方面同样敏感（分别为89.5%和91.9%）且特异性极高（100%）。与DIB（68%）相比，ELISA在检测ALA患者循环中的阿米巴抗原方面更敏感（94.4%）。ELISA和DIB法分别在3例和5例AD患者中检测到了显著水平的特异性抗体。由于DIB法操作更简便且成本更低，因此推荐用于检测侵袭性阿米巴病患者的抗体。
