Gandhi B M
Trans R Soc Trop Med Hyg. 1986;80(6):883-5. doi: 10.1016/0035-9203(86)90246-4.
A rapid, cheap, simple and specific serological test of adequate sensitivity for detecting IgG antibodies against Entamoeba histolytica antigen is described. Axenically cultured amoebic antigen was used to precoat the nitrocellulose membrane. The strips were incubated with test samples and later with horseradish peroxidase (HRPO) labelled protein-A conjugate. A dark blue spot was obtained by treatment with peroxidase substrate, 4-chloro-1-naphthol, in positive samples. Serum samples from 32 healthy controls, 45 patients with acute amoebic liver abscess and 10 asymptomatic E. histolytica cyst passers were tested. This test was positive in 93% of cases of amoebic liver abscess, 3% of healthy controls and none of the cyst passers; its sensitivity (97%) and specificity (93%) were as good as that of the ELISA test and, because it is simple, quick and cheap it is recommended as a serological test of choice for the diagnosis of invasive amoebiasis.
本文描述了一种快速、廉价、简单且特异的血清学检测方法，该方法具有足够的灵敏度，可用于检测抗溶组织内阿米巴抗原的IgG抗体。采用无共生培养的阿米巴抗原预包被硝酸纤维素膜。将试纸条与测试样品孵育，随后与辣根过氧化物酶（HRPO）标记的蛋白A缀合物孵育。阳性样品经过氧化物酶底物4-氯-1-萘酚处理后会出现深蓝色斑点。对32名健康对照者、45例急性阿米巴肝脓肿患者和10例无症状溶组织内阿米巴包囊携带者的血清样本进行了检测。该检测方法在93%的阿米巴肝脓肿病例中呈阳性，在3%的健康对照者中呈阳性，在所有包囊携带者中均为阴性；其灵敏度（97%）和特异性（93%）与ELISA检测相当，且由于其简单、快速和廉价，推荐作为诊断侵袭性阿米巴病的血清学首选检测方法。
