López Revilla R, Navarro-García F, Valadez-Sánchez M, López Vidal Y, Calva Mercado J
Departamento de Biología Celular, CINVESTAV-IPN, México, D.F.
Arch Invest Med (Mex). 1991 Jul-Dec;22(3-4):249-53.
Seroepidemiologic and clinical studies of amoebiasis require improved methods for the detection and titration of anti-Entamoeba histolytica (Eh) trophozoite antibodies. We have developed a Dot-enzyme-linked immunosorbent assay (Dot-ELISA) to titrate anti-Eh antibodies in human serum and colostrum. The anti-Eh serum IgG antibody titers from healthy individuals and from patients with amoebic liver abscess obtained, with our Dot-ELISA were similar to those obtained with a standard ELISA, but the background values were lower. Significant anti-Eh sIgA antibody titers were found in seven out of 42 colostrum samples of mothers of newborn children with diarrhea. Our Dot-ELISA is rapid, inexpensive, and might be used for the diagnosis of individuals and in seroepidemiologic studies of amoebiasis.
阿米巴病的血清流行病学和临床研究需要改进检测和滴定抗溶组织内阿米巴（Eh）滋养体抗体的方法。我们开发了一种斑点酶联免疫吸附测定法（Dot-ELISA）来滴定人血清和初乳中的抗Eh抗体。通过我们的Dot-ELISA获得的健康个体和阿米巴肝脓肿患者的抗Eh血清IgG抗体滴度与标准ELISA获得的滴度相似，但背景值更低。在42例腹泻新生儿母亲的初乳样本中，有7例发现了显著的抗Eh sIgA抗体滴度。我们的Dot-ELISA快速、廉价，可用于个体诊断和阿米巴病的血清流行病学研究。
