Alam Mohammad Zahangir, Haralambous Christos, Kuhls Katrin, Gouzelou Evi, Sgouras Dionyssios, Soteriadou Ketty, Schnur Lionel, Pratlong Francine, Schönian Gabriele
Institut für Mikrobiologie und Hygiene, Charité Universitätsmedizin Berlin, Berlin, Germany.
Microbes Infect. 2009 May-Jun;11(6-7):707-15. doi: 10.1016/j.micinf.2009.04.009. Epub 2009 Apr 17.
Multilocus microsatellite typing (MLMT) was employed to compare strains of Leishmania donovani belonging to the MON-37 zymodeme (MON-37 strains) from Cyprus and Israel to MON-37 strains from the Indian subcontinent, the Middle East, China and East Africa as well as strains of other zymodemes. The MLMT data were processed with a distance-based method for construction of phylogenetic trees, factorial correspondence analysis and a Bayesian model-based clustering algorithm. All three approaches assigned the MON-37 strains to different distantly related genetically defined subgroups, corresponding to their geographical origin. Specifically, the Kenyan, Sri Lankan and Indian MON-37 strains were genetically closer to strains of other zymodemes from the same regions than to MON-37 strains from other areas. MON-37 strains from Cyprus and Israel were clearly different not only among themselves, but also compared to all the other MON-37 strains studied and could, therefore, be autochthonous. This study showed that the zymodeme MON-37 is paraphyletic and does not reflect the genetic relationship between strains of different geographical origin.
采用多位点微卫星分型（MLMT）方法，将来自塞浦路斯和以色列的属于MON - 37酶谱型（MON - 37菌株）的杜氏利什曼原虫菌株与来自印度次大陆、中东、中国和东非的MON - 37菌株以及其他酶谱型的菌株进行比较。利用基于距离的方法处理MLMT数据，以构建系统发育树、因子对应分析和基于贝叶斯模型的聚类算法。所有这三种方法都将MON - 37菌株归为不同的、亲缘关系较远的基因定义亚组，这与它们的地理起源相对应。具体而言，肯尼亚、斯里兰卡和印度的MON - 37菌株在基因上与来自相同地区的其他酶谱型菌株更为接近，而非与来自其他地区的MON - 37菌株接近。来自塞浦路斯和以色列的MON - 37菌株不仅彼此明显不同，而且与所有其他研究的MON - 37菌株相比也存在差异，因此可能是本地起源的。这项研究表明，酶谱型MON - 37是并系的，不能反映不同地理起源菌株之间的遗传关系。
