Olsina Kissler Jorge Juan, Balsells Valls Joaquín, Dot Bach Juan, Suboh Abadia Monder Abu, Armengol Bertroli Jordi, Esteves Marielle, Rosal Fontana Marta, Armengol-Miró José Ramón
World Institute for Digestive Endoscopy Research, WIDER-Barcelona (colaboración Obra Social Fundación La Caixa), Barcelona, España.
Cir Esp. 2009 May;85(5):298-306. doi: 10.1016/j.ciresp.2008.11.007. Epub 2009 Apr 18.
The advent of natural orifice endoscopic surgery (NOTES) and new prototypes for performing this surgical procedure led us to design an experimental animal surgical programme.
NOTES was performed over a period of one year, in sows, following the European guidelines on the use of experimental animals. Ninety operations were performed with no animals surviving. The following aspects were assessed: 1. Access route complexity (transgastric, transvaginal, transesophageal and transumbilical). 2. Support measures for temporal/spatial orientation. 3. Technical possibilities for visceral orifice closure (clips, T-bars, Obesco clips and endoscopic suture). Resections of fallopian tubes, ovaries, gallbladder, mediastinal lymph nodes, tail of the pancreas and gastrojejunal derivations were performed with one or two endoscopes.
This experience enabled us to highlight a series of technical aspects essential for these techniques: 1. Pneumoperitoneum with CO(2) is safer for entry. 2. Orifice size is important to limit contamination. 3. Puncture entry and guided dilation is safer. 4. Good gastric exit location makes it easier to approach viscera to be resected. 5. Intra-abdominal haemorrhage is difficult to control. 6. Leak-free closure cannot be guaranteed with clips, but t-bars and Obesco clips may be effective. 7. Endoscopes that permit triangulation may facilitate the dissection and endoscopic suture.
NOTES requires a multidisciplinary team comprising laparoscopic and endoscopic surgeons. Pure NOTES is complex and hybrid forms or transumbilical route could be intermediate steps.
自然腔道内镜手术(NOTES)的出现以及用于实施该手术的新原型促使我们设计了一项实验动物外科手术方案。
按照欧洲关于实验动物使用的指南,在母猪身上进行了为期一年的NOTES手术。共进行了90例手术,无动物存活。评估了以下几个方面:1. 进入途径的复杂性(经胃、经阴道、经食管和经脐)。2. 时间/空间定位的支持措施。3. 内脏孔闭合的技术可能性(夹子、T形夹、Obesco夹和内镜缝合)。使用一或两个内镜进行输卵管、卵巢、胆囊、纵隔淋巴结、胰尾和胃空肠改道术的切除。
该经验使我们能够突出这些技术所必需的一系列技术要点:1. 使用CO₂建立气腹用于进入更安全。2. 孔的大小对于限制污染很重要。3. 穿刺进入和引导扩张更安全。4. 良好的胃出口位置便于接近要切除的内脏。5. 腹腔内出血难以控制。6. 夹子不能保证无渗漏闭合,但T形夹和Obesco夹可能有效。7. 允许三角定位的内镜可能有助于解剖和内镜缝合。
NOTES需要一个由腹腔镜和内镜外科医生组成的多学科团队。单纯的NOTES很复杂,混合形式或经脐途径可能是中间步骤。