Blanco Enrique, Abril Josep F
Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Spain.
Methods Mol Biol. 2009;537:243-61. doi: 10.1007/978-1-59745-251-9_12.
The sequence of many eukaryotic genomes is nowadays available from a personal computer to any researcher in the world-wide scientific community. However, the sequences are worthless without the adequate annotation of the biological meaningful elements. The annotation of the genes, in particular, is a challenging task that can not be tackled without the aid of specific bioinformatics tools. We present in this chapter a simple protocol mainly based on the combination of the program GeneID and other computational tools to annotate the location of a gene, which was previously annotated in D. melanogaster, in the recently assembled genome of D. yakuba.
如今，许多真核生物基因组序列从个人电脑就能获取，全球科学界的任何研究人员都能使用。然而，如果没有对具有生物学意义的元件进行充分注释，这些序列就毫无价值。尤其是基因注释，这是一项具有挑战性的任务，没有特定生物信息学工具的帮助就无法完成。在本章中，我们提出了一个简单的方案，主要基于GeneID程序和其他计算工具的组合，来注释一个先前在黑腹果蝇中注释过的基因在新组装的雅库布果蝇基因组中的位置。
