Khaja Razi, MacDonald Jeffrey R, Zhang Junjun, Scherer Stephen W
Program in Genetics and Genomic Biology, Research Institute, The Hospital for Sick Children, Toronto, ON, Canada.
Methods Mol Biol. 2006;338:9-20. doi: 10.1385/1-59745-097-9:9.
The aim of this chapter is to provide instruction for analyzing and mapping recent segmental and gene duplications in eukaryotic genomes. We describe a bioinformatics-based approach utilizing computational tools to manage eukaryotic genome sequences to characterize and understand the evolutionary fates and trajectories of duplicated genes. An introduction to bioinformatics tools and programs such as BLAST, Perl, BioPerl, and the GFF specification provides the necessary background to complete this analysis for any eukaryotic genome of interest.
本章旨在提供关于分析和绘制真核生物基因组中近期片段重复和基因重复的指导。我们描述了一种基于生物信息学的方法，利用计算工具来管理真核生物基因组序列，以表征和理解重复基因的进化命运和轨迹。对诸如BLAST、Perl、BioPerl和GFF规范等生物信息学工具和程序的介绍，为完成对任何感兴趣的真核生物基因组的这一分析提供了必要的背景知识。
