Chatterjee Diptendu, Gerlai Robert
Department of Psychology, University of Toronto, Mississauga, Ontario, Canada.
Behav Brain Res. 2009 Jun 8;200(1):208-13. doi: 10.1016/j.bbr.2009.01.016.
Zebrafish is gaining popularity in behavioral neuroscience in general and in alcohol research in particular. Alcohol is known to affect numerous molecular mechanisms depending on dose and administration regimen. Prominent among these mechanisms are several neurotransmitter systems. Here we analyze the responses of the dopaminergic and serotoninergic neurotransmitter systems of zebrafish to acute alcohol treatment (1 h long exposure of adult fish to 0.00%, 0.25%, 0.50%, or 1.00% ethyl alcohol) by testing the concentration of dopamine, its metabolite DOPAC, and serotonin and its metabolite 5-HIAA from whole brain extracts. We utilize a sensitive HPLC method and describe significant alcohol induced changes in zebrafish for the first time. We show that dopamine significantly increased in a quasi-linear dose dependent manner, DOPAC showed a smaller apparent increase which was non-significant, while both serotonin and 5-HIAA showed a significant increase only in the highest acute dose group. We discuss the methodological novelty of our work and theorize about the implications of the neurotransmitter level changes from a behavioral perspective.
斑马鱼在行为神经科学领域，尤其是在酒精研究中越来越受到关注。众所周知，酒精会根据剂量和给药方案影响众多分子机制。其中突出的机制包括几个神经递质系统。在此，我们通过检测全脑提取物中多巴胺、其代谢物DOPAC、血清素及其代谢物5-HIAA的浓度，分析斑马鱼的多巴胺能和血清素能神经递质系统对急性酒精处理（成年鱼暴露于0.00%、0.25%、0.50%或1.00%乙醇中1小时）的反应。我们采用了一种灵敏的高效液相色谱法，并首次描述了酒精对斑马鱼产生的显著变化。我们发现，多巴胺以近似线性的剂量依赖性方式显著增加，DOPAC的增加幅度较小且不显著，而血清素和5-HIAA仅在最高急性剂量组中显著增加。我们讨论了我们工作的方法新颖性，并从行为学角度对神经递质水平变化的影响进行了理论探讨。
