Delgado Luz, Schmachtenberg Oliver
CNV, Universidad de Valparaíso, Avda. Gran Bretaña 1111, Casilla 5029, Correo 4, 2360102 Valparaíso, Chile.
Cerebellum. 2008;7(3):444-50. doi: 10.1007/s12311-008-0047-7.
Gamma-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter in the central nervous system. Its role is especially prominent in the cerebellum, where most neuron types are GABAergic. However, little is known about its function in the cerebellum of teleost fish, which is only partly homologous to its mammalian counterpart. Here, we investigated the expression and distribution of GABA, the GABA-synthesizing enzyme glutamic acid decarboxylase 65 (GAD65), and the receptor subunits GABA(Aalpha1) and GABA(B1) in the cerebellum of adult zebrafish. GABA and GAD65 presented a similar expression pattern that comprised the molecular layer, Purkinje cells and groups of presumed Golgi cells in the granular layer, both in the cerebellar corpus and valve. GABA(A) receptor subunits are principally found on fine radial fibers in the molecular layer, while GABA(B) receptor subunits localized prominently to the cell bodies of Purkinje cells in the ganglionic layer, and to their dendrites that span the molecular layer. These results are compared to the expression of the GABAergic system in the mammalian cerebellum.
γ-氨基丁酸（GABA）是中枢神经系统中的主要抑制性神经递质。其作用在小脑尤为突出，在小脑中大多数神经元类型都是γ-氨基丁酸能的。然而，对于硬骨鱼小脑的功能知之甚少，硬骨鱼小脑仅部分与哺乳动物的小脑同源。在此，我们研究了成年斑马鱼小脑中GABA、GABA合成酶谷氨酸脱羧酶65（GAD65）以及受体亚基GABA（Aα1）和GABA（B1）的表达和分布。GABA和GAD65呈现出相似的表达模式，包括分子层、浦肯野细胞以及颗粒层中假定的高尔基细胞群，在小脑体和小脑瓣中均如此。GABA（A）受体亚基主要存在于分子层的细放射状纤维上，而GABA（B）受体亚基主要定位于神经节层浦肯野细胞的细胞体及其跨越分子层的树突上。将这些结果与哺乳动物小脑中γ-氨基丁酸能系统的表达进行了比较。
