Choi Eun Mi, Koo Sung Ja, Hwang Jae-Kwan
Department of Biotechnology and Bioproducts Research Center, Yonsei University, Shinchon-dong 134, Seodaemoon-gu, Seoul 120-749, South Korea.
J Ethnopharmacol. 2004 Mar;91(1):1-6. doi: 10.1016/j.jep.2003.11.006.
Mucopolysaccharide (YMP) of yam (Dioscorea batatas Decne) was tested for immunomodulatory activity in vitro. We examined the effect of YMP on the viability, cell-mediated cytotoxicity and IFN-gamma secretion of splenic lymphocytes. The cytotoxic activity of mouse splenocyte against leukemia cell was increased in the presence of YMP (10 microg/ml). However, YMP (10 microg/ml) did not affect the viability of splenocytes. The production of IFN-gamma was significantly increased in the YMP treated splenocytes, suggesting that YMP may induce cell-mediated immune responses. The effects of YMP on phagocytic activity were studied in relation to uptaking capacity and lysosomal phosphatase activity of the mouse peritoneal macrophages in vitro. YMP (50 microg/ml) was found to increase uptaking capacity and lysosomal phosphatase activity of peritoneal macrophages. In addition, YMP (10-100 microg/ml) significantly increased the viability of peritoneal macrophages (P<0.05). The data obtained demonstrate that the YMP may be used as sources of immunoactive polysaccharides.
对山药（薯蓣）中的粘多糖（YMP）进行了体外免疫调节活性测试。我们研究了YMP对脾淋巴细胞活力、细胞介导的细胞毒性及γ干扰素分泌的影响。在存在YMP（10微克/毫升）的情况下，小鼠脾细胞对白血病细胞的细胞毒性活性增强。然而，YMP（10微克/毫升）不影响脾细胞的活力。YMP处理的脾细胞中γ干扰素的产生显著增加，表明YMP可能诱导细胞介导的免疫反应。体外研究了YMP对小鼠腹腔巨噬细胞吞噬活性的影响，涉及摄取能力和溶酶体磷酸酶活性。发现YMP（50微克/毫升）可增加腹腔巨噬细胞的摄取能力和溶酶体磷酸酶活性。此外，YMP（10 - 100微克/毫升）显著提高了腹腔巨噬细胞的活力（P<0.05）。所获得的数据表明，YMP可用作免疫活性多糖的来源。
