通过病毒空斑和感染性测定揭示的抗体依赖性细胞对单纯疱疹病毒传播的保护作用。
Antibody-dependent cellular protection against herpes simplex virus dissemination as revealed by viral plauqe and infectivity assays.
作者信息
Shimizu F, Hanaumi K, Shimizu Y, Kumagai K
出版信息
Infect Immun. 1977 May;16(2):531-6. doi: 10.1128/iai.16.2.531-536.1977.
Mouse nonimmune peripheral blood lymphocytes (PBL) plus antibody to herpes simplex virus inhibited virus dissemination in herpes simplex virus-infected 3T3 cell culture as revealed by development of viral plaque, size of immunofluorescent foci, appearance of polycaryocytes, and viral infectivity appearing in the culture. These nonimmune PBL did not act alone in inibiting virus dissemination, but did act synergistically in combination with antibody. The ratio of PBL to target monolayer cells needed to produce this effect was 20. Splenic lymphocytes had weak activity, whereas thymocytes were without effect, even in the presence of antibody. Neither interferon nor lymphotoxin was detected in this lymphocyte-mediated response. These findings support previous observations, based on cytotoxicity assays, that antibody-dependent cellular immune processes could be important in control of and recovery from herpesvirus infection.
小鼠非免疫外周血淋巴细胞（PBL）加抗单纯疱疹病毒抗体可抑制单纯疱疹病毒在3T3细胞培养物中的传播，这可通过病毒蚀斑的形成、免疫荧光灶的大小、多核细胞的出现以及培养物中出现的病毒感染性来揭示。这些非免疫PBL并非单独发挥抑制病毒传播的作用，而是与抗体协同发挥作用。产生这种效应所需的PBL与靶单层细胞的比例为20。脾淋巴细胞活性较弱，而胸腺细胞即使在有抗体存在的情况下也无作用。在这种淋巴细胞介导的反应中未检测到干扰素或淋巴毒素。这些发现支持了基于细胞毒性试验的先前观察结果，即抗体依赖性细胞免疫过程在疱疹病毒感染的控制和恢复中可能很重要。
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