Zaia J A, Palmer E L, Feorino P M
J Infect Dis. 1975 Dec;132(6):660-6. doi: 10.1093/infdis/132.6.660.
The humoral and cellular immune responses of rabbits and guinea pigs to the envelope-associated antigen of herpes simplex virus type I were studied. Neutralizing antibody (at high titer) and lymphocytes reactive to herpes simplex virus were detected in both guinea pigs and rabbits after immunization with the antigen. In a standard assay of cellular immunity to herpes simplex virus, the antigen stimulated blast transformation of herpes simplex virus-reactive splenic lymphocytes in vitro. Furthermore, immunization of rabbits with the envelope-associated antigen protected the animals from a lethal dose of live herpes simplex virus. Thus an antigen of herpes simplex virus can be prepared which contains neither infectious nor noninfectious viral particles and which stimulates immunity to the virus in laboratory animals.
对兔和豚鼠针对I型单纯疱疹病毒包膜相关抗原的体液免疫和细胞免疫反应进行了研究。在用该抗原免疫后，在豚鼠和兔中均检测到（高滴度的）中和抗体以及对单纯疱疹病毒有反应的淋巴细胞。在针对单纯疱疹病毒的细胞免疫标准检测中，该抗原在体外刺激了对单纯疱疹病毒有反应的脾淋巴细胞的母细胞转化。此外，用包膜相关抗原免疫兔可保护动物免受致死剂量的活单纯疱疹病毒感染。因此，可以制备一种单纯疱疹病毒抗原，其既不包含传染性也不包含非传染性病毒颗粒，并且能在实验动物中刺激对该病毒的免疫反应。
