Wang Wen, Sun Fangling, An Yi, Ai Houxi, Zhang Li, Huang Wenting, Li Lin
Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing, China.
Eur J Pharmacol. 2009 Jun 24;613(1-3):19-23. doi: 10.1016/j.ejphar.2009.04.013. Epub 2009 Apr 18.
Oxidative stress-induced cell damage has been implicated in a variety of neurodegenerative disorders. Morroniside, an iridorid glycoside isolated from Cornus officinalis Sieb. Et Zucc., has shown potent antioxidant properties. The present study investigated the protective actions of morroniside against the cytotoxicity produced by exposure to H(2)O(2) (300-500 microM) in SH-SY5Y cells. Intracellular accumulation of Ca(2+), and decreases in mitochondrial membrane potential (MMP) caused by added H(2)O(2) were reduced by morroniside. Incubation of cells with H(2)O(2) caused a marked decrease in superoxide dismutase (SOD) activity; this decrease was significantly inhibited by morroniside. In addition, the percentage of cells undergoing H(2)O(2)-induced apoptosis was decreased, dose dependently, in the presence of morroniside. These results suggest that morroniside has protective effects against oxidative stress-induced neurotoxic processes.
氧化应激诱导的细胞损伤与多种神经退行性疾病有关。山茱萸苷是从山茱萸中分离出的一种环烯醚萜苷，已显示出强大的抗氧化特性。本研究调查了山茱萸苷对SH-SY5Y细胞暴露于H₂O₂（300 - 500微摩尔）所产生的细胞毒性的保护作用。山茱萸苷可减少由添加的H₂O₂引起的细胞内Ca²⁺积累以及线粒体膜电位（MMP）的降低。用H₂O₂孵育细胞会导致超氧化物歧化酶（SOD）活性显著降低；山茱萸苷可显著抑制这种降低。此外，在存在山茱萸苷的情况下，H₂O₂诱导的细胞凋亡百分比呈剂量依赖性降低。这些结果表明，山茱萸苷对氧化应激诱导的神经毒性过程具有保护作用。
