Wang Wen, Huang Wenting, Li Lin, Ai Houxi, Sun Fangling, Liu Ci, An Yi
Department of Pharmacology, Xuan-Wu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing, 100053, China.
Cell Mol Neurobiol. 2008 Feb;28(2):293-305. doi: 10.1007/s10571-007-9168-7. Epub 2007 Jul 24.
(1) Morroniside belongs to an extensive group of natural iridorid glycosides. In the present study, using human neuroblastoma SH-SY5Y cells, we have investigated the protective effects of this compound on modifications in endogenous reduced glutathione (GSH), intracellular oxygen species (ROS) and apoptotic death on H(2)O(2)-mediated cytoxicity. (2) Incubation of cells with morroniside led to a significant dose-dependent elevation of cellular GSH accompanied by a marked protection against H(2)O(2)-mediated toxicity. Morroniside at 1-100 microM inhibited the formation of ROS and the activation of caspase-3 and 9, and the upregulation of Bcl-2, whereas no significant change occurred in Bax levels. (3) The results indicated that the anti-oxidative and anti-apoptotic properties render this natural compound potentially protective against H(2)O(2)-induced cytotoxicity. (4) This study suggested that intracellular GSH appeared to be an important factor in morroniside-mediated cytoprotection against H(2)O(2)-toxicity in SH-SY5Y cells.
(1) 莫诺苷属于一大类天然环烯醚萜苷。在本研究中，我们使用人神经母细胞瘤SH-SY5Y细胞，研究了该化合物对H₂O₂介导的细胞毒性作用下内源性还原型谷胱甘肽（GSH）修饰、细胞内活性氧（ROS）及凋亡死亡的保护作用。(2) 用莫诺苷孵育细胞导致细胞内GSH显著剂量依赖性升高，并对H₂O₂介导的毒性有显著保护作用。1-100微摩尔的莫诺苷抑制了ROS的形成以及半胱天冬酶-3和9的激活，上调了Bcl-2，而Bax水平无显著变化。(3) 结果表明，这种天然化合物的抗氧化和抗凋亡特性使其对H₂O₂诱导的细胞毒性具有潜在保护作用。(4) 本研究表明，细胞内GSH似乎是莫诺苷介导的对SH-SY5Y细胞中H₂O₂毒性的细胞保护作用的一个重要因素。
