苦楝叶中的甾体及其对人癌细胞系的细胞毒性作用。
Steroids from the leaves of Chinese Melia azedarach and their cytotoxic effects on human cancer cell lines.
作者信息
Wu Shi-Biao, Ji Yan-Ping, Zhu Jing-Jing, Zhao Yun, Xia Gang, Hu Ying-He, Hu Jin-Feng
机构信息
Department of Natural Products for Chemical Genetic Research, Key Laboratory of Brain Functional Genomics, Ministry of Education, East China Normal University, Shanghai, PR China.
出版信息
Steroids. 2009 Sep;74(9):761-5. doi: 10.1016/j.steroids.2009.04.005. Epub 2009 Apr 18.
Three new (1-3) and several known (4-6) steroids were isolated from the leaves of Chinese Melia azedarach. The structures of the new compounds were elucidated by means of spectroscopic methods including 2D NMR techniques and mass spectrometry to be (20S)-5,24(28)-ergostadiene-3beta,7alpha,16beta,20-tetrol (1), (20S)-5-ergostene-3beta,7alpha,16beta,20-tetrol (2), and 2alpha,3beta-dihydro-5-pregnen-16-one (3). The cytotoxicities of the isolated compounds against three human cancer cell lines (A549, H460, U251) were evaluated; only compounds 1, 2, and (20S)-5-stigmastene-3beta,7alpha,20-triol (4) were found to show significant cyctotoxic effects with IC(50)s from 12.0 to 30.1 microg/mL.
从苦楝叶中分离出三种新的甾体化合物（1 - 3）以及几种已知的甾体化合物（4 - 6）。通过包括二维核磁共振技术和质谱在内的光谱方法阐明了新化合物的结构，分别为（20S）-5,24(28)-麦角甾二烯-3β,7α,16β,20-四醇（1）、（20S）-5-麦角甾烯-3β,7α,16β,20-四醇（2）和2α,3β-二氢-5-孕烯-16-酮（3）。评估了分离出的化合物对三种人类癌细胞系（A549、H460、U251）的细胞毒性；仅发现化合物1、2和（20S）-5-豆甾烯-3β,7α,20-三醇（4）具有显著的细胞毒性作用，半数抑制浓度（IC50）为12.0至30.1μg/mL。
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