Center for the Theoretical Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
Biomed Microdevices. 2009 Oct;11(5):981-6. doi: 10.1007/s10544-009-9315-7. Epub 2009 Apr 21.
We present a simple and high-throughput microfluidic system for diffusion-based monolayer yeast cell culture monitoring. Yeast cells are patterned into the micro-cavity array with a suitable height (4 μm) that keeps the cells fixed in monolayer during the cell division. Different sizes of cavities and different repeating times of injection were tested in order to realize as many single-cell/cavity as possible. Single-cell/cavity has been achieved in about 40% of 100 parallel cavities. As a demonstration, we apply this technology to investigate budding yeast and fission yeast cultures and show that it permits single-cell resolution over many cellular generations. Our results show that the technique provides an easy way to study the phenotype of single yeast cell cycle or cell-cell communication in high-throughput microfluidic system.
我们提出了一种简单且高通量的基于扩散的单层酵母细胞培养监测微流控系统。通过合适的高度（4μm）将酵母细胞图案化成微腔阵列，使细胞在细胞分裂过程中保持单层固定。为了实现尽可能多的单细胞/腔，我们测试了不同大小的腔和不同的注射重复次数。在 100 个平行腔中，约有 40%实现了单细胞/腔。作为演示，我们将这项技术应用于研究出芽酵母和裂殖酵母培养物，并表明它可以在多个细胞世代中实现单细胞分辨率。我们的结果表明，该技术为在高通量微流控系统中研究单个酵母细胞周期或细胞间通讯的表型提供了一种简单的方法。
