Frey Olivier, Rudolf Fabian, Schmidt Gregor W, Hierlemann Andreas
ETH Zürich, †Department of Biosystems Science and Engineering, Bio Engineering Laboratory, and ‡Department of Biosystems Science and Engineering, Computational Systems Biology Group, Mattenstrasse 26, 4058 Basel, Switzerland.
Anal Chem. 2015 Apr 21;87(8):4144-51. doi: 10.1021/ac504611t. Epub 2015 Apr 10.
Optical long-term observation of individual cells, combined with modern data analysis tools, allows for a detailed study of cell-to-cell variability, heredity, and differentiation. We developed a microfluidic device featuring facile cell loading, simple and robust operation, and which is amenable to high-resolution life-cell imaging. Different cell strains can be grown in parallel in the device under constant or changing media perfusion without cross-talk between the cell ensembles. The culturing chamber has been optimized for use with nonadherent cells, such as Saccharomyces cerevisiae, and enables controlled colony growth over multiple generations under aerobic or anaerobic conditions. Small changes in the layout will make the device also useable with bacteria or mammalian cells. The platform can be readily set up in every laboratory with minimal additional requirements and can be operated without technology training.
对单个细胞进行长期光学观察，并结合现代数据分析工具，能够对细胞间变异性、遗传性和分化进行详细研究。我们开发了一种微流控装置，其具有易于细胞加载、操作简单且稳健，并且适用于高分辨率活细胞成像的特点。不同的细胞株可以在该装置中在恒定或变化的培养基灌注条件下并行生长，细胞群体之间不会相互干扰。培养室已针对非贴壁细胞（如酿酒酵母）进行了优化，并且能够在需氧或厌氧条件下实现多代受控菌落生长。布局上的微小改变将使该装置也可用于细菌或哺乳动物细胞。该平台可以在每个实验室以最少的额外要求轻松搭建，并且无需技术培训即可操作。
