一种候选的减毒活牛冠状病毒疫苗：安全性和免疫原性评估。
A candidate modified-live bovine coronavirus vaccine: safety and immunogenicity evaluation.
作者信息
Decaro Nicola, Campolo Marco, Mari Viviana, Desario Costantina, Colaianni Maria Loredana, Di Trani Livia, Cordioli Paolo, Buonavoglia Canio
机构信息
Department of Public Health and Animal Sciences, Faculty of Veterinary Medicine of Bari, Strada per Casamassima km 3, 70010 Valenzano, Bari, Italy.
出版信息
New Microbiol. 2009 Jan;32(1):109-13.
A modified-live vaccine against the respiratory form of bovine coronavirus (BCoV) infection was developed by progressive attenuation of a respiratory strain (438/06-TN). The vaccine was found to be safe as four colostrum-deprived newborn calves remained healthy after oronasal administration of ten doses of the vaccine. The immunogenicity of the vaccine was assessed by intramuscular injection of one vaccine dose to 30 BCoV-antibody negative 2-3-month-old calves. At 30 days post-vaccination, all vaccinated calves displayed high antibody titres against BCoV. Sequence analysis of the S gene of wild-type and cell-adapted 438/06-TN strain detected 10 nucleotide changes, 9 of which were non-synonymous.
通过对一株呼吸道毒株（438/06-TN）进行逐步减毒，研发出了一种针对牛冠状病毒（BCoV）呼吸道感染形式的减毒活疫苗。经发现，该疫苗是安全的，因为4头初乳剥夺的新生犊牛在经口鼻腔接种10剂疫苗后仍保持健康。通过对30头2-3月龄BCoV抗体阴性的犊牛肌肉注射一剂疫苗来评估疫苗的免疫原性。在接种疫苗后30天，所有接种疫苗的犊牛均表现出针对BCoV的高抗体滴度。对野生型和细胞适应型438/06-TN毒株的S基因进行序列分析，检测到10个核苷酸变化，其中9个为非同义变化。
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