Li Q, Huang W, Zhou X
Department of Pathology, Clinical School of Medical College of Nanjing University, Nanjing Jinling Hospital, Nanjing, China.
J Int Med Res. 2009 Mar-Apr;37(2):446-54. doi: 10.1177/147323000903700220.
This retrospective study investigated CD34, alpha-smooth muscle actin (alpha-SMA), and transforming growth factor-beta1 (TGF-beta1) expression in stromal cells of cervical intraepithelial neoplasias (CINs; n = 30), invasive cervical squamous cell carcinomas (SCCs; n = 38) and adjacent normal cervix. Normal cervix and CINs contained diffuse CD34-positive stromal cells but no alpha-SMA-positive myofibroblasts. In contrast, 34 of 38 SCCs were free of CD34-positive stromal cells and all contained alpha-SMA-positive stromal myofibroblasts; adjacent normal tissue contained CD34-positive stromal cells and no alpha-SMA-positive myofibroblasts. More intense TGF-beta1 expression was observed in SCC cells than in normal cervical epithelium or CINs. This study shows that the disappearance of CD34-positive stromal cells and appearance of alpha-SMA-positive stromal myofibroblasts may be associated with transformation of cervical CIN to SCC. These findings support the suggestion that over-production of TGF-beta1 in SCC cells is one potential mechanism mediating the transformation of stromal cells to myofibroblasts in cervical carcinogenesis.
这项回顾性研究调查了30例宫颈上皮内瘤变（CIN）、38例浸润性宫颈鳞状细胞癌（SCC）及相邻正常宫颈基质细胞中CD34、α-平滑肌肌动蛋白（α-SMA）和转化生长因子-β1（TGF-β1）的表达情况。正常宫颈和CIN含有弥漫性CD34阳性基质细胞，但无α-SMA阳性肌成纤维细胞。相比之下，38例SCC中有34例无CD34阳性基质细胞，且均含有α-SMA阳性基质肌成纤维细胞；相邻正常组织含有CD34阳性基质细胞，无α-SMA阳性肌成纤维细胞。SCC细胞中观察到的TGF-β1表达比正常宫颈上皮或CIN中更强烈。本研究表明，CD34阳性基质细胞的消失和α-SMA阳性基质肌成纤维细胞的出现可能与宫颈CIN向SCC的转变有关。这些发现支持了以下观点，即SCC细胞中TGF-β1的过度产生是介导宫颈癌发生过程中基质细胞向肌成纤维细胞转化的一种潜在机制。
